DATASHEET
Host:
Rabbit
Target Protein:
Bestrophin
Immunogen Range:
251-350/585
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
5068
Source:
KLH conjugated synthetic peptide derived from human Bestrophin
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Best vitelliform macular dystrophy, known as Best disease, is an early-onset autosomal dominant condition in which accumulation of lipofuscin-like material within and beneath the RPE leads to progressive loss of central vision. Best disease is frequently a reflection of mutations in the Bestrophin gene, which encodes a protein containing four putative transmembrane domains and localizes to the basolateral plasma membrane of RPE cells. Human Bestrophin forms oligomeric chloride channels that are sensitive to intracellular calcium. Missense mutations at the Bestrophin locus reduces or abolishes Bestrophin protein mediated membrane current. Bestrophin Bestrophin 2,Bestrophin 3, and Bestrophin 4 are transmembrane proteins that contain a high percentage of aromatic residues, a conserved RFP (Arg-Phe-Pro) motif and they function as anion channels.
PRODUCT SPECIFIC PUBLICATIONS
- Deng Y et al. High SPRR1A expression is associated with poor survival in patients with colon cancer. Oncol Lett. 2020 May;19(5):3417-3424. Read more>>
- Deng Y et al. High SPRR1A expression is associated with poor survival in patients with colon cancer. Oncol Lett. 2020 May;19(5):3417-3424. Read more>>
- Amirkavei M et al. Induction of Heat Shock Protein 70 in Mouse RPE as an In Vivo Model of Transpupillary Thermal Stimulation. Int J Mol Sci. 2020 Mar 17;21(6). pii: E2063.Read more>>
- Liu, Haitao. et al. Reducing Akt2 in retinal pigment epithelial cells causes a compensatory increase in Akt1 and attenuates diabetic retinopathy. NAT COMMUN. 2022 Oct;13(1):1-19Read more>>
VALIDATION IMAGES
Antigen: bs-11040P, 2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \nTMB staining\nRead the data in Microplate Reader by 450nm. \n