bs-1110R [Primary Antibody]
Osterix Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Osterix

Immunogen Range: 331-431/431


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 121340

Swiss Prot: Q8TDD2

Source: KLH conjugated synthetic peptide derived from human Osterix

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 45


Cross Reactive Species: Human
Mouse
Rabbit

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Jin‐Ran, et al. "Diet derived phenolic acids regulate osteoblast and adipocyte lineage commitment and differentiation in young mice." Journal of Bone and Mineral Research (2013).Read more>>
  • Dong, Weijie, et al. "Phenotypic characterization of craniofacial bone marrow stromal cells: unique properties of enhanced osteogenesis, cell recruitment, autophagy, and apoptosis resistance." Cell and Tissue Research (2014): 1-11.Read more>>
  • Kishi, Yuta, et al. "PARP Inhibitor PJ34 Suppresses Osteogenic Differentiation in Mouse Mesenchymal Stem Cells by Modulating BMP-2 Signaling Pathway."International Journal of Molecular Sciences 16.10 (2015): 24820-24838.Read more>>
  • Agarwal, Shailesh, et al. "Analysis of bone‐cartilage‐stromal progenitor populations in trauma induced and genetic models of heterotopic ossification." STEM CELLS (2016).Read more>>
  • Tang, Zhurong, et al. "Comparison of ectopic bone formation process induced by four calcium phosphate ceramics in mice." Materials Science and Engineering: C (2016).Read more>>
  • Schlie-Wolter, S., et al. Osteogenic Differentiation of Human Adipose-Derived Stem Cells (hASC): Role of FAK and Ras-MAPK Signaling. (2016) J Stem Cell Regen Bio 2(2): 1- 19Read more>>
  • Agarwal, Shailesh, et al. "Local and Circulating Endothelial Cells Undergo Endothelial to Mesenchymal Transition (EndMT) in Response to Musculoskeletal Injury." Scientific Reports 6 (2016): 32514.Read more>>
  • Rajshankar, Dhaarmini, Yongqiang Wang, and Christopher A. McCulloch. "Osteogenesis requires FAK-dependent collagen synthesis by fibroblasts and osteoblasts." The FASEB Journal (2016): fj-201600645R.Read more>>
  • Wang, Yilin, et al. "Synergistic effects of overexpression of BMP‑2 and TGF‑β3 on osteogenic differentiation of bone marrow mesenchymal stem cells." Molecular Medicine Reports (2016): 11.Read more>>
  • Ranganathan, Kavitha, et al. "The role of the adaptive immune system in burn-induced heterotopic ossification and mesenchymal cell osteogenic differentiation." Journal of Surgical Research 206.1 (2016): 53-61.Read more>>
  • Li, Shao, et al. "A Conditional Knockout Mouse Model Reveals a Critical Role of PKD1 in Osteoblast Differentiation and Bone Development." Scientific Reports 7 (2017): 40505.Read more>>
  • Qian, Dong-Yang, et al. "Differential circRNA expression profiles during the BMP2-induced osteogenic differentiation of MC3T3-E1 cells." Biomedicine & Pharmacotherapy 90 (2017): 492-499.Read more>>
  • Shi, Hua-Li, et al. "Effect of polygonimitin C on bone formation and resorption in human osteoblast-like MG63 cells." Tropical Journal of Pharmaceutical Research 16.7 (2017): 1595-1600.Read more>>
  • Chen et al. Diet derived phenolic acids regulate osteoblast and adipocyte lineage commitment and differentiation in young mice. (2013) J.Bone.Miner.Res. Read more>>
  • Agarwal et al. Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. (2016) Stem.Cells. 34:1692-701Read more>>
  • Bihari C et al. Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy. (2018) Hepatol Commun.Sep 4;2(9):1095-1110.Read more>>
  • Cui Q et al. Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway. Cell Death Dis. 2019 Feb 20;10(3):172. Read more>>
  • Chang Z et al. Reticulocalbin 2 enhances osteogenic differentiation of human vascular smooth muscle cells in diabetic conditions. Life Sci. 2019 Sep 15;233:116746.Read more>>
  • Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039Read more>>
  • Yongkui Wang. et al. Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3/-catenin signaling. Toxicol Appl Pharm. 2020 Dec;409:115333Read more>>
  • o Zhang. et al. Neuropeptide?Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3?E1 cells via an autocrine mechanism. Mol Med Rep. 2020 Nov;22(5):4376-4382Read more>>
  • Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 MaRead more>>
  • Yu-Ting Yen. et al. PP2A in LepR+ mesenchymal stem cells contributes to embryonic and postnatal endochondral ossification through Runx2 dephosphorylation. Commun Biol. 2021 Jun;4(1):1-12Read more>>
  • Kohei Arakawa. et al. The difference in joint instability affects the onset of cartilage degeneration or subchondral bone changes. Osteoarthr Cartilage. 2021 DecRead more>>
  • Zhao, Gang. et al. LINC02381, a sponge of miR-21, weakens osteogenic differentiation of hUC-MSCs through KLF12-mediated Wnt4 transcriptional repression. 2021 Nov 15Read more>>
  • Rui Zhang. et al. Multifunctional silicon calcium phosphate composite scaffolds promote stem cell recruitment and bone regeneration. J MATER CHEM B. 2022 JuRead more>>
  • Song Xiaoliang. et al. Bioinspired Protein/Peptide Loaded 3D Printed PLGA Scaffold Promotes Bone Regeneration. FRONT BIOENG BIOTECH. 2022 Jul;0:1195Read more>>
  • Jiarui Lu. et al. Injectable Col-_/CS hydrogel enhances bone regeneration in mice tibial mono-cortical defect with impaired osteogenesis. MATER TODAY COMMUN. 2022 Aug;32:10407Read more>>
  • Jingyuan Wen. et al. Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects. BIOMED PHARMACOTHER. 2023 Feb;158:114122Read more>>
  • ?brahim Halilullah Erbay. et al. Bioengineering bone-on-a-chip model harnessing osteoblastic and osteoclastic resolution. ADV ENG MATER. 2022 DecRead more>>
  • Xiaoxia Hao. et al. Bone deterioration in response to chronic high-altitude hypoxia is attenuated by a pulsed electromagnetic field via the primary cilium/HIF-1 axis. J BONE MINER RES. 2023 JaRead more>>
  • Hongrong Zhang. et al. Extracellular matrix of ameloblastoma-derived negatively regulates osteogenic differentiation. ORAL DIS. 2023 JulRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Osterix Polyclonal Antibody, Unconjugated (bs-1110R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse spinal cord lysates probed with Osterix Polyclonal Antibody, unconjugated (bs-1110R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Mouse Embryo tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse Cerebrum tissue lysates probed with SP7/Osterix Polyclonal Antibody, Unconjugated (bs-1110R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.