bs-1111R [Primary Antibody]
Oct-4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: 4-Oct

Immunogen Range: 201-300/360


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18999

Swiss Prot: P20263

Source: KLH conjugated synthetic peptide derived from mouse OCT-4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 39


Cross Reactive Species: Human
Mouse
Rat
Others

Predicted Cross Reactive Species: Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang EX et al. Stem cells from trabecular meshwork cells can secrete extracellular matrix. Biochem Biophys Res Commun. 2020 Mar 5;523(2):522-526.Read more>>
  • Wang EX et al. Stem cells from trabecular meshwork cells can secrete extracellular matrix. Biochem Biophys Res Commun. 2020 Mar 5;523(2):522-526.Read more>>
  • Mahmoud Labibet al. Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. Nat Biomed Eng. 2020 Jul 27.Read more>>
  • Ya Fang Wuet al. Growth and Stem Cell Characteristics of Tendon-Derived Cells with Different Initial Seeding Densities: An In Vitro Study in MouseFlexor Tendon Cells. Stem Cells Dev. 2020 Aug 1;29(15):1016-1025.Read more>>
  • Jiaming Fu. et al. mTORC1 coordinates NF-_B signaling pathway to promote chondrogenic differentiation of tendon cells in heterotopic ossification. BONE. 2022 Oct;163:116507Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse tooth germ labeled with Anti-Oct-3/Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


L1 rat brain lysates L2 rat heart lysates probed with Anti Oct-3/Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 42kD. Observed band size:42kD.\n


Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Oct-4 Polyclonal Antibody, Unconjugated (bs-1111R) at 1:200 overnight at 4°C, DAB staining.