bs-11182R-A555 [Conjugated Primary Antibody]
EMR1 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: EMR1

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human EMR1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (1–3). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (1–3). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1,3,4). The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5,6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages.

Conjugation: ALEXA FLUOR® 555

Excitation/ Emission: 555nm/580nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang M et al. The role of TrkA in the promoting wounding-healing effect of CD271 on epidermal stem cells. (2018) Arch. Dermatol. Res.Read more>>
  • Jiang X et al.Loss of MD1 exacerbates myocardial ischemia/reperfusion injury and susceptibility to ventricular arrhythmia.(2018)Eur J Pharmacol;844:79-86. Read more>>
  • Yang R et al. Cordycepin Attenuates IFN-γ-Induced Macrophage IP-10 and Mig Expressions by Inhibiting STAT1 Activity in CFA-Induced Inflammation Mice Model. Inflammation. 2019 Dec 23.Read more>>
  • An G et al. Effects of CCL5 on the biological behavior of breast cancer and the mechanisms of its interaction with tumor‑associated macrophages. Oncol Rep. 2019 Dec;42(6):2499-2511. Read more>>
  • Li T et al. Withanolides, extracted from Datura metel L. inhibit keratinocyte proliferation and imiquimod- induced psoriasis-like dermatitis via the STAT3/P38/ERK1/2 pathway. Molecules. 2019 Jul 17;24(14). pii: E2596. Read more>>
  • Liu X et al. KDM3A inhibition modulates macrophage polarization to aggravate post-MI injuries and accelerates adverse ventricular remodeling via an IRF4 signaling pathway. Cell Signal. 2019 Sep 9;64:109415.Read more>>
  • Tang Y et al. Overcoming the Reticuloendothelial System Barrier to Drug Delivery with a "Don't-Eat-Us" Strategy. ACS Nano. 2019 Nov 5. Read more>>
  • Sun Y et al. Apple polysaccharide prevents from colitis-associated carcinogenesis through regulating macrophage polarization. Int J Biol Macromol. 2020 Oct 15;161:704-711.Read more>>
  • Liu S et al. Absorbable Thioether Grafted Hyaluronic Acid Nanofibrous Hydrogel for Synergistic Modulation of Inflammation Microenvironment to Accelerate Chronic Diabetic Wound Healing. Adv Healthc Mater. 2020 Apr 27:e2000198.Read more>>
  • Quanyu Chen. et al. Hepatocyte growth factor mediates a novel form of hepatic stem/progenitor cell-induced tolerance in a rat xenogeneic liver rejection model. Int Immunopharmacol. 2021 Jan;90:10718Read more>>
  • Sha Liu. et al. Dual Cross-linked HHA Hydrogel Supplies and Regulates M2 for Synergistic Improvement of Immunocompromise and Impaired Angiogenesis to Enhance Diabetic Chronic Wound Healing. Biomacromolecules. 2020;21(9):3795C3806Read more>>
  • Gaoyang Guo. et al. A method for simultaneously crosslinking and functionalizing extracellular matrix-based biomaterials as bioprosthetic heart valves with enhanced endothelialization and reduced inflammation. Acta Biomater. 2021 Jan;119:89Read more>>
  • Jiejuan Lai. et al. Comparison of the biological and functional characteristics of mesenchymal stem cells from intrahepatic and identical bone marrow. Stem Cell Res. 2021 Aug;55:102477Read more>>
  • Xu Jun Yan. et al. Interleukin-5-induced eosinophil population improves cardiac function after myocardial infarction. CARDIOVASC RES. 2022 Jul;118(9):2165-2178Read more>>
  • Xie, Jian. et al. Aligned electrospun poly(l-lactide) nanofibers facilitate wound healing by inhibiting macrophage M1 polarization via the JAK-STAT and NF-_B pathways. J NANOBIOTECHNOL. 2022 Dec;20(1):1-16Read more>>
  • Xiong, Jingjie. et al. CircRNA mmu_circ_0000021 regulates microvascular function via the miR-143-3p/NPY axis and intracellular calcium following ischemia/reperfusion injury. CELL DEATH DISCOV. 2022 Jul;8(1):1-13Read more>>
  • Chao Huang. et al. Loss of TRIM67 Attenuates the Progress of Obesity-Induced Non-Alcoholic Fatty Liver Disease. INT J MOL SCI. 2022 Jan;23(13):7475Read more>>
  • Yifan Wang. et al. Dulaglutide Ameliorates Intrauterine Adhesion by Suppressing Inflammation and EpithelialCMesenchymal Transition via Inhibiting the TGF-/Smad2 Signaling Pathway. PHARMACEUTICALS-BASE. 2023 Jul;16(7):964Read more>>
VALIDATION IMAGES