Host: Rabbit
Target Protein: KLC1/KNS2
Immunogen Range: 1-100/573
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3831
Swiss Prot: Q07866
Source: KLH conjugated synthetic peptide derived from human KLC1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 65
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Sheep
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-KLC1/KNS2 Polyclonal Antibody, Unconjugated (bs-11212R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Lane 1: Hela lysates Lane 2:293T lysates probed with Rabbit Anti-KLC1/KNS2 Polyclonal Antibody, Unconjugated (bs-11212R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Rat Placenta tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human MCF-7 cell lysates; Lane 8: Human HeLa cell lysates probed with KLC1 Polyclonal Antibody, Unconjugated (bs-11212R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.