bs-11227R [Primary Antibody]
Microcephalin 1/BRIT1 Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: Microcephalin 1/BRIT1

Immunogen Range: 11-110/835


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human Microcephalin 1/BRIT1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Microcephalin modulates brain size and has been proliferating under strong positive selection for several thousand years, although the nature of the positive selection is poorly understood. Human Microcephalin contains three BRCA1 C-terminal (BRCT) domains and shares 57% identity with its mouse ortholog, the most conserved regions being BRCT domains where there is 80% identity. Predominant expression of human Microcephalin is observed in fetal brain, liver and kidney tissues and is expressed during neurogenesis in mice. Microcephalin displays significantly higher rates of protein evolution in primates than in rodents; this trend is most noticeable for the subset of genes associated with nervous system development. Microcephalin has a very young, single nucleotide, polymorphism haplotype associated with modern humans; this gene is presumably still evolving in Homo sapiens. It functions in DNA damage response and regulation of cell cycle checkpoints.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 93


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Dog

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human K562 cell lysates probed with Microcephalin 1/BRIT1 Polyclonal Antibody, Unconjugated (bs-11227R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.