bs-1122R [Primary Antibody]
Sema3F/semaphorin 3F Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Sema3F/semaphorin 3F

Immunogen Range: 651-754/754


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6405

Swiss Prot: Q13275

Source: KLH conjugated synthetic peptide derived from human Sema3F

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues(Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 88


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ni, Qianwei, et al. "The neuropilins and their ligands in hematogenous metastasis from salivary adenoid cystic carcinoma-an immunohistochemical study." Journal of Oral and Maxillofacial Surgery (2017).Read more>>
  • Se Eun Ha. et al. Transcriptome profiling of subepithelial PDGFR_ cells in colonic mucosa reveals several cell-selective markers. PLOS ONE. 2022 May;17(5):e0261743Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Sema3F/semaphorin 3F Polyclonal Antibody, Unconjugated (bs-1122R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded rat spleen labeled with Anti-Sema3F/semaphorin 3F Polyclonal Antibody, Unconjugated (bs-1122R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Paraformaldehyde-fixed, paraffin-embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Sema3F/semaphorin 3F Polyclonal Antibody, Unconjugated (bs-1122R) at 1:200 overnight at 4°C, DAB staining.