bs-1130R [Primary Antibody]
Gemin 2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Gemin 2

Immunogen Range: 51-150/280


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8487

Swiss Prot: O14893

Source: KLH conjugated synthetic peptide derived from human SIP1/Gemin 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 30


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat colon labeled with Anti-Gemin 2 Polyclonal Antibody, Unconjugated (bs-1130R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Gemin 2 Polyclonal Antibody, Unconjugated(bs-1130R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Alexa Fluor 488 conjugated(bs-0295G-AF488)used at 1:200 dilution for 40 minutes at 37°C.


Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human MOLT4 cell lysates probed with Gemin 2 Polyclonal Antibody, Unconjugated (bs-1130R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.