Host: Rabbit
Target Protein: Neuroglycan C
Immunogen Range: 351-450/566
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 10675
Swiss Prot: O95196
Source: KLH conjugated synthetic peptide derived from human Neuroglycan C
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 57
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Neuroglycan Polyclonal Antibody, Unconjugated (bs-11323R) at 1:300 overnight at 4°C, followed by a conjugated secondary and DAB staining\n
Human U251 cells probed with Neuroglycan C Polyclonal Antibody, unconjugated (bs-11323R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n
Mouse cerebrum lysates probed with Neuroglycan C Polyclonal Antibody, unconjugated (bs-11323R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n