Host: Rabbit
Target Protein: SPARC
Immunogen Range: 101-200/303
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6678
Swiss Prot: P09486
Source: KLH conjugated synthetic peptide derived from human SPARC
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 33
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Chen, Jie, et al. "SPARC is a key regulator of proliferation, apoptosis and invasion in human ovarian cancer." PLoS One 7.8 (2012): e42413.Read more>>
- Kurtul, Neslihan, et al. "Prognostic Value of SPARC Expression in Unresectable NSCLC Treated with Concurrent Chemoradiotherapy." Asian Pacific Journal of Cancer Prevention 15.20 (2014): 8911-8916.Read more>>
- Zong, Shaohui, et al. "Effects of Polygonatum sibiricum polysaccharide on the osteogenic differentiation of bone mesenchymal stem cells in mice." International Journal of Clinical and Experimental Pathology8.6 (2015): 6169-6180.Read more>>
- Liting Cheng. et al. Bioresponsive micro-to-nano albumin-based systems for targeted drug delivery against complex fungal infections. Acta Pharm Sin B. 2021 MayRead more>>
- Zheng, Qingbo. et al. Construction of transcriptome atlas of white yak hair follicle during anagen and catagen using single-cell RNA sequencing. BMC GENOMICS. 2022 Dec;23(1):1-14Read more>>
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-SPARC Polyclonal Antibody, Unconjugated (bs-1133R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SPARC Polyclonal Antibody, Unconjugated (bs-1133R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SPARC Polyclonal Antibody, Unconjugated (bs-1133R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SPARC Polyclonal Antibody, Unconjugated (bs-1133R) at 1:500 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml) was used to stain the cell nuclei
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-SPARC Polyclonal Antibody, Unconjugated (bs-1133R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse brain lysates probed with SPARC Polyclonal Antibody, Unconjugated (bs-1133R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A549 cell lysates probed with SPARC Polyclonal Antibody, Unconjugated (bs-1133R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SPARC Polyclonal Antibody, Unconjugated (bs-1133R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SPARC Polyclonal Antibody, Unconjugated (bs-1133R) at 1:200 overnight at 4°C, DAB staining.