bs-11348R [Primary Antibody]
Hippocalcin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Hippocalcin

Immunogen Range: 101-193/193


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human Hippocalcin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Hippocalcin is a neuron-specific calcium-binding protein found primarily in the plasma membrane of brain and retinal tissue, with increased expression observed in hippocampal pyramidal cells. Through its calcium-dependent signal regulation, hippocalcin can both inhibit rhodopsin kinase and increase phospholipase D2 expression. In order to regulate kinase and phospholipase activity, hippocalcin must bind to the plasma membrane where it can then bind two calcium ions for use in signal regulation. The hippocalcin protein is highly conserved in mouse, rat and human tissue and has a suggested role in neural plasticity and associative memory by contributing to the survival of neurons during aging. The loss of hippocalcin expression is thought to contribute to age-related impairment of post-synaptic functions related to neuronal degradation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 22


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Cow
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Cerebral cortex lysates probed with Hippocalcin Polyclonal Antibody, Unconjugated (bs-11348R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Hippocampus lysates probed with Hippocalcin Polyclonal Antibody, Unconjugated (bs-11348R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.