bs-1134R
[Primary Antibody]
RUNX1 + RUNX2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: RUNX1+RUNX2
Immunogen Range: 202-300/521
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 860
Swiss Prot: Q13950
Source: KLH conjugated synthetic peptide derived from human RUNX2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 57
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Guinea Pig
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Tang Y et al. Structure and ingredient-based biomimetic scaffolds combining with autologous bone marrow-derived mesenchymal stem cell sheets for bone-tendon healing. Biomaterials. 2020 May;241:119837.Read more>>
- Hu, Fei, et al. "High expression of periostin is dramatically associated with metastatic potential and poor prognosis of patients with osteosarcoma." World Journal of Surgical Oncology 12.1 (2014): 287.Read more>>
- Li, Pengcui, et al. "Blockade of hypoxia-induced CXCR4 with AMD3100 inhibits production of OA-associated catabolic mediators IL-1β and MMP-13." Molecular Medicine Reports.Read more>>
- Sun et al. The role of inhibition by phosphocitrate and its analogue in chondrocyte differentiation and subchondral bone advance in Hartley guinea pigs. (2018) Exp.Ther.Med. 15:3320-3328Read more>>
- Lu H et al. Comparative evaluation of book‐type acellular bone scaffold and fibrocartilage scaffold for bone‐tendon healing. J Orthop Res. 2019 Apr 12. Read more>>
- "Chen X et al. Modulation of proliferation and differentiation of gingiva‑derived mesenchymal stem cells by concentrated growth factors: Potential implications in tissue engineering for dental regeneration and repair. Send to Int J Mol Med. 2019 Apr 24. "Read more>>
- Li Z et al. Angiogenesis and bone regeneration by allogeneic mesenchymal stem cell intravenous transplantation in rabbit model of avascular necrotic femoral head. J Surg Res. 2013 Jul;183(1):193-203. Read more>>
- Xu Y et al. Silver nanoparticles promote osteogenic differentiation of human periodontal ligamentfibroblasts by regulating the RhoA-TAZ axis. Cell Biol Int. 2019 May 22. Read more>>
- Li Y et al. Sulfonated chitosan oligosaccharide alleviates the inhibitory effect of basic fibroblast growth factor on osteogenic differentiation of human periodontal ligament stem cells. J Periodontol. 2019 Oct 1.Read more>>
- Li M et al. Comparison of bone surface and trough fixation on bone–tendon healing in a rabbit patella–patellar tendon injury model. Journal of Orthopaedic Translation. 2020.Read more>>
- Tang Y et al. Structure and ingredient-based biomimetic scaffolds combining with autologous bone marrow-derived mesenchymal stem cell sheets for bone-tendon healing. Biomaterials. 2020 May;241:119837.Read more>>
- Zhang X et al. Ginsenoside Rg3 attenuates ovariectomy\induced osteoporosis via AMPK/mTOR signaling pathway. Drug Dev Res. 2020 Nov;81(7):875-884.Read more>>
- Liu M et al. Luminescent Net-like Inorganic Scaffolds with Europium Doped Hydroxyapatite for Enhanced Bone ReconstructionNanoscale.2021 Jan 6.Read more>>
- Xijuan Liu et al. Chondrocyte suppression is mediated by miR\129\5p via GDF11/SMAD3 signaling in developmental dysplasia of the hip. J Orthop Res. 2020 Dec;38(12):2559-2572.Read more>>
- Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12Read more>>
- Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039Read more>>
- Jilong Zou. et al. Resveratrol Benefits the Lineage Commitment of BMSCs into Osteoblasts via MiR\320c by Targeting Runx2. 2021 Jan 22Read more>>
- Liang Ai. et al. Xian-Ling-Gu-Bao protects osteoporosis through promoting osteoblast differentiation by targeting miR-100-5p/KDM6B/RUNX2 axis. In Vitro Cell Dev-An. 2021 Jan;57(1):3-9Read more>>
- Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 MaRead more>>
- Shiyi Huang. et al. Transforming Growth Factor-3/Recombinant Human-like Collagen/Chitosan Freeze-Dried Sponge Primed With Human Periodontal Ligament Stem Cells Promotes Bone Regeneration in Calvarial Defect Rats. Front Pharmacol. 2021; 12: 678322Read more>>
- Yi Liu. et al. Modification of COL1A1 in Autologous Adipose Tissue-Derived Progenitor Cells Rescues the Bone Phenotype in a Mouse Model of Osteogenesis Imperfecta. 2021 May 18Read more>>
- Shan Shan Qi. et al. Lycopene ameliorates diabetic osteoporosis via anti-inflammatory, anti-oxidation, and increasing Osteoprotegerin/RANKL expression ratio. J Funct Foods. 2021 Aug;83:104539Read more>>
- Zhang, Zilong. et al. Circ_FBLN1 promotes the proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by regulating let-7i-5p/FZD4 axis and Wnt/-catenin pathway. 2021 Aug 23Read more>>
- Ming Ni. et al. Sox11 Modified Tendon-Derived Stem Cells Promote the Repair of Osteonecrosis of Femoral Head:. Cell Transplant. 2021;()Read more>>
- Yanyan Xing. et al. Effects of mechanosensitive ion channel Piezo1 on proliferation and osteogenic differentiation of human dental follicle cells. Ann Anat. 2021 Oct;:151847Read more>>
- Yang Sun. et al. Dual Biosignal-Functional Injectable Microspheres for Remodeling Osteogenic Microenvironment. SMALL. 2022 Apr 14Read more>>
- Lingli Ding. et al. Ginsenoside Compound K Enhances Fracture Healing <i>via</i> Promoting Osteogenesis and Angiogenesis.. FRONT PHARMACOL. 2022 Apr;13:855393-855393Read more>>
- L Cheng. et al. Toxic effects of thioacetamide-induced femoral damage in New Zealand white rabbits by activating the p38/ERK signaling pathway. PHYSIOL RES. 2022 Apr 11Read more>>
- Tongtong Zhu. et al. Construction and validation of steroid-induced rabbit osteonecrosis model. MethodsX. 2022 Apr;:101713Read more>>
- Lu, Gonggong. et al. An instantly fixable and self-adaptive scaffold for skull regeneration by autologous stem cell recruitment and angiogenesis. NAT COMMUN. 2022 May;13(1):1-2Read more>>
- Junchao Huang. et al. Palovarotene inhibits the NF-_B signaling pathway to prevent heterotopic ossification. CLIN EXP PHARMACOL P. 2022 May 31Read more>>
- Rui Zhang. et al. Multifunctional silicon calcium phosphate composite scaffolds promote stem cell recruitment and bone regeneration. J MATER CHEM B. 2022 JuRead more>>
- Peiyang Gu. et al. Tailorable 3DP Flexible Scaffolds with Porosification of Filaments Facilitate Cell Ingrowth and Biomineralized Deposition. ACS APPL MATER INTER. 2022;XXXX(XXX):XXX-XXXRead more>>
- Ning Liu. et al. Bu-Gu-Sheng-Sui decoction promotes osteogenesis <i>via</i> activating the ERK/Smad signaling pathways.. FRONT PHARMACOL. 2022 Aug;13:976121-976121Read more>>
- Zhifa Wang. et al. The combination of a 3D-Printed porous TiC6AlC4V alloy scaffold and stem cell sheet technology for the construction of biomimetic engineered bone at an ectopic site. MATER TODAY BIO. 2022 Dec;16:100433Read more>>
- Fanglin Wang. et al. Adipose-derived stem cells with miR-150-5p inhibition laden in hydroxyapatite/tricalcium phosphate ceramic powders promote osteogenesis via regulating Notch3 and activating FAK/ERK and RhoA. ACTA BIOMATER. 2022 OcRead more>>
- Zhaxi Mima. et al. Blockade of JAK2 retards cartilage degeneration and IL-6-induced pain amplification in osteoarthritis. INT IMMUNOPHARMACOL. 2022 Dec;113:10934Read more>>
- Lin, Xiaoxin. et al. Shikonin promotes rat periodontal bone defect repair and osteogenic differentiation of BMSCs by p38 MAPK pathway. ODONTOLOGY. 2022 Dec;:1-9Read more>>
- Kai Wang. et al. Network Pharmacological Analysis and Animal Experimental Study on Osteoporosis Treatment with GuBen-ZengGu Granules. EVID-BASED COMPL ALT. 2023 Jan 12;2023:9317557Read more>>
- Yingjie Duan. et al. VX-765 ameliorates CKD VSMC calcification by regulating STAT3 activation. EUR J PHARMACOL. 2023 Apr;945:17561Read more>>
- Yixin Xia. et al. Regulation of endothelial cells on the osteogenic ability of bone marrow mesenchymal stem cells in peri-implantitis. TISSUE CELL. 2023 Apr;81:102042Read more>>
- Yi-Xuan Wang. et al. MiR-20a promotes osteogenic differentiation in bone marrow-derived mesenchymal stem/stromal cells and bone repair of the maxillary sinus defect model in rabbits. FRONT BIOENG BIOTECH. 2023; 11: 1127908Read more>>
- Feng Xiao. et al. Identification of TNF-mediated inflammation as potential pathological marker and therapeutic target for calcification progress of congenital bicuspid aortic valve. EUR J PHARMACOL. 2023 Jul;951:175783Read more>>
- Changjun Chen. et al. Engineered Exosome-Functionalized Extracellular Matrix-Mimicking Hydrogel for Promoting Bone Repair in Glucocorticoid-Induced Osteonecrosis of the Femoral Head. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
- Juan Yan. et al. Engineered exosomes reprogram Gli1+ cells in vivo to prevent calcification of vascular grafts and autologous pathological vessels. SCI ADV. 2023 Jul;9(29)Read more>>
- Li Fu-Xing-Zi. et al. Cold exposure protects against medial arterial calcification development via autophagy. J NANOBIOTECHNOL. 2023 Dec;21(1):1-22Read more>>
- Hongrong Zhang. et al. Extracellular matrix of ameloblastoma-derived negatively regulates osteogenic differentiation. ORAL DIS. 2023 JulRead more>>
- Xishuai Tong. et al. Vitamin D Alleviates Cadmium-Induced Inhibition of Chicken Bone Marrow Stromal Cells Osteogenic Differentiation In Vitro. ANIMALS. 2023 Jan;13(15):2544Read more>>
- Zheng Ming-Hui. et al. Vascular wall microenvironment: exosomes secreted by adventitial fibroblasts induced vascular calcification. J NANOBIOTECHNOL. 2023 Dec;21(1):1-21Read more>>
- Lin Xu. et al. Local injection of abaloparatide promotes mandibular condyle lengthening in adolescent rats via enhancing chondrogenesis and ossification. J ORAL REHABIL. 2023 SepRead more>>
VALIDATION IMAGES
Lane 1: HL60 cell lysates; Lane 2: Mouse testis lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates;Lane 2: Rat Cerebrum lysates;Lane 3: Mouse heart lysates; Lane 4: Rat heart lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RUNX1 + RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:400 overnight at 4°C, DAB staining.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RUNX1 + RUNX2 Polyclonal Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Lymph node lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Colon tissue lysates; Lane 2: Mouse Lymph node tissue lysates; Lane 3: Rat Colon tissue lysates; Lane 4: Rat Lymph node tissue lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.