bs-1134R-HRP [Conjugated Primary Antibody]
RUNX1 + RUNX2 Polyclonal Antibody, HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RUNX1+RUNX2

Immunogen Range: 202-300/521


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 860

Swiss Prot: Q13950

Source: KLH conjugated synthetic peptide derived from human RUNX2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 57


Cross Reactive Species: Human
Mouse
Rat
Rabbit
Guinea Pig

Predicted Cross Reactive Species: Dog
Cow
Sheep
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tang Y et al. Structure and ingredient-based biomimetic scaffolds combining with autologous bone marrow-derived mesenchymal stem cell sheets for bone-tendon healing. Biomaterials. 2020 May;241:119837.Read more>>
  • Hu, Fei, et al. "High expression of periostin is dramatically associated with metastatic potential and poor prognosis of patients with osteosarcoma." World Journal of Surgical Oncology 12.1 (2014): 287.Read more>>
  • Li, Pengcui, et al. "Blockade of hypoxia-induced CXCR4 with AMD3100 inhibits production of OA-associated catabolic mediators IL-1β and MMP-13." Molecular Medicine Reports.Read more>>
  • Sun et al. The role of inhibition by phosphocitrate and its analogue in chondrocyte differentiation and subchondral bone advance in Hartley guinea pigs. (2018) Exp.Ther.Med. 15:3320-3328Read more>>
  • Lu H et al. Comparative evaluation of book‐type acellular bone scaffold and fibrocartilage scaffold for bone‐tendon healing. J Orthop Res. 2019 Apr 12. Read more>>
  • "Chen X et al. Modulation of proliferation and differentiation of gingiva‑derived mesenchymal stem cells by concentrated growth factors: Potential implications in tissue engineering for dental regeneration and repair. Send to Int J Mol Med. 2019 Apr 24. "Read more>>
  • Li Z et al. Angiogenesis and bone regeneration by allogeneic mesenchymal stem cell intravenous transplantation in rabbit model of avascular necrotic femoral head. J Surg Res. 2013 Jul;183(1):193-203. Read more>>
  • Xu Y et al. Silver nanoparticles promote osteogenic differentiation of human periodontal ligamentfibroblasts by regulating the RhoA-TAZ axis. Cell Biol Int. 2019 May 22. Read more>>
  • Li Y et al. Sulfonated chitosan oligosaccharide alleviates the inhibitory effect of basic fibroblast growth factor on osteogenic differentiation of human periodontal ligament stem cells. J Periodontol. 2019 Oct 1.Read more>>
  • Li M et al. Comparison of bone surface and trough fixation on bone–tendon healing in a rabbit patella–patellar tendon injury model. Journal of Orthopaedic Translation. 2020.Read more>>
  • Tang Y et al. Structure and ingredient-based biomimetic scaffolds combining with autologous bone marrow-derived mesenchymal stem cell sheets for bone-tendon healing. Biomaterials. 2020 May;241:119837.Read more>>
  • Zhang X et al. Ginsenoside Rg3 attenuates ovariectomy\induced osteoporosis via AMPK/mTOR signaling pathway. Drug Dev Res. 2020 Nov;81(7):875-884.Read more>>
  • Liu M et al. Luminescent Net-like Inorganic Scaffolds with Europium Doped Hydroxyapatite for Enhanced Bone ReconstructionNanoscale.2021 Jan 6.Read more>>
  • Xijuan Liu et al. Chondrocyte suppression is mediated by miR\129\5p via GDF11/SMAD3 signaling in developmental dysplasia of the hip. J Orthop Res. 2020 Dec;38(12):2559-2572.Read more>>
  • Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12Read more>>
  • Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039Read more>>
  • Jilong Zou. et al. Resveratrol Benefits the Lineage Commitment of BMSCs into Osteoblasts via MiR\320c by Targeting Runx2. 2021 Jan 22Read more>>
  • Liang Ai. et al. Xian-Ling-Gu-Bao protects osteoporosis through promoting osteoblast differentiation by targeting miR-100-5p/KDM6B/RUNX2 axis. In Vitro Cell Dev-An. 2021 Jan;57(1):3-9Read more>>
  • Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 MaRead more>>
  • Shiyi Huang. et al. Transforming Growth Factor-3/Recombinant Human-like Collagen/Chitosan Freeze-Dried Sponge Primed With Human Periodontal Ligament Stem Cells Promotes Bone Regeneration in Calvarial Defect Rats. Front Pharmacol. 2021; 12: 678322Read more>>
  • Yi Liu. et al. Modification of COL1A1 in Autologous Adipose Tissue-Derived Progenitor Cells Rescues the Bone Phenotype in a Mouse Model of Osteogenesis Imperfecta. 2021 May 18Read more>>
  • Shan Shan Qi. et al. Lycopene ameliorates diabetic osteoporosis via anti-inflammatory, anti-oxidation, and increasing Osteoprotegerin/RANKL expression ratio. J Funct Foods. 2021 Aug;83:104539Read more>>
  • Zhang, Zilong. et al. Circ_FBLN1 promotes the proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by regulating let-7i-5p/FZD4 axis and Wnt/-catenin pathway. 2021 Aug 23Read more>>
  • Ming Ni. et al. Sox11 Modified Tendon-Derived Stem Cells Promote the Repair of Osteonecrosis of Femoral Head:. Cell Transplant. 2021;()Read more>>
  • Yanyan Xing. et al. Effects of mechanosensitive ion channel Piezo1 on proliferation and osteogenic differentiation of human dental follicle cells. Ann Anat. 2021 Oct;:151847Read more>>
  • Yang Sun. et al. Dual Biosignal-Functional Injectable Microspheres for Remodeling Osteogenic Microenvironment. SMALL. 2022 Apr 14Read more>>
  • Lingli Ding. et al. Ginsenoside Compound K Enhances Fracture Healing <i>via</i> Promoting Osteogenesis and Angiogenesis.. FRONT PHARMACOL. 2022 Apr;13:855393-855393Read more>>
  • L Cheng. et al. Toxic effects of thioacetamide-induced femoral damage in New Zealand white rabbits by activating the p38/ERK signaling pathway. PHYSIOL RES. 2022 Apr 11Read more>>
  • Tongtong Zhu. et al. Construction and validation of steroid-induced rabbit osteonecrosis model. MethodsX. 2022 Apr;:101713Read more>>
  • Lu, Gonggong. et al. An instantly fixable and self-adaptive scaffold for skull regeneration by autologous stem cell recruitment and angiogenesis. NAT COMMUN. 2022 May;13(1):1-2Read more>>
  • Junchao Huang. et al. Palovarotene inhibits the NF-_B signaling pathway to prevent heterotopic ossification. CLIN EXP PHARMACOL P. 2022 May 31Read more>>
  • Rui Zhang. et al. Multifunctional silicon calcium phosphate composite scaffolds promote stem cell recruitment and bone regeneration. J MATER CHEM B. 2022 JuRead more>>
  • Peiyang Gu. et al. Tailorable 3DP Flexible Scaffolds with Porosification of Filaments Facilitate Cell Ingrowth and Biomineralized Deposition. ACS APPL MATER INTER. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Ning Liu. et al. Bu-Gu-Sheng-Sui decoction promotes osteogenesis <i>via</i> activating the ERK/Smad signaling pathways.. FRONT PHARMACOL. 2022 Aug;13:976121-976121Read more>>
  • Zhifa Wang. et al. The combination of a 3D-Printed porous TiC6AlC4V alloy scaffold and stem cell sheet technology for the construction of biomimetic engineered bone at an ectopic site. MATER TODAY BIO. 2022 Dec;16:100433Read more>>
  • Fanglin Wang. et al. Adipose-derived stem cells with miR-150-5p inhibition laden in hydroxyapatite/tricalcium phosphate ceramic powders promote osteogenesis via regulating Notch3 and activating FAK/ERK and RhoA. ACTA BIOMATER. 2022 OcRead more>>
  • Zhaxi Mima. et al. Blockade of JAK2 retards cartilage degeneration and IL-6-induced pain amplification in osteoarthritis. INT IMMUNOPHARMACOL. 2022 Dec;113:10934Read more>>
  • Lin, Xiaoxin. et al. Shikonin promotes rat periodontal bone defect repair and osteogenic differentiation of BMSCs by p38 MAPK pathway. ODONTOLOGY. 2022 Dec;:1-9Read more>>
  • Kai Wang. et al. Network Pharmacological Analysis and Animal Experimental Study on Osteoporosis Treatment with GuBen-ZengGu Granules. EVID-BASED COMPL ALT. 2023 Jan 12;2023:9317557Read more>>
  • Yingjie Duan. et al. VX-765 ameliorates CKD VSMC calcification by regulating STAT3 activation. EUR J PHARMACOL. 2023 Apr;945:17561Read more>>
  • Yixin Xia. et al. Regulation of endothelial cells on the osteogenic ability of bone marrow mesenchymal stem cells in peri-implantitis. TISSUE CELL. 2023 Apr;81:102042Read more>>
  • Yi-Xuan Wang. et al. MiR-20a promotes osteogenic differentiation in bone marrow-derived mesenchymal stem/stromal cells and bone repair of the maxillary sinus defect model in rabbits. FRONT BIOENG BIOTECH. 2023; 11: 1127908Read more>>
  • Feng Xiao. et al. Identification of TNF-mediated inflammation as potential pathological marker and therapeutic target for calcification progress of congenital bicuspid aortic valve. EUR J PHARMACOL. 2023 Jul;951:175783Read more>>
  • Changjun Chen. et al. Engineered Exosome-Functionalized Extracellular Matrix-Mimicking Hydrogel for Promoting Bone Repair in Glucocorticoid-Induced Osteonecrosis of the Femoral Head. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Juan Yan. et al. Engineered exosomes reprogram Gli1+ cells in vivo to prevent calcification of vascular grafts and autologous pathological vessels. SCI ADV. 2023 Jul;9(29)Read more>>
  • Li Fu-Xing-Zi. et al. Cold exposure protects against medial arterial calcification development via autophagy. J NANOBIOTECHNOL. 2023 Dec;21(1):1-22Read more>>
  • Hongrong Zhang. et al. Extracellular matrix of ameloblastoma-derived negatively regulates osteogenic differentiation. ORAL DIS. 2023 JulRead more>>
  • Xishuai Tong. et al. Vitamin D Alleviates Cadmium-Induced Inhibition of Chicken Bone Marrow Stromal Cells Osteogenic Differentiation In Vitro. ANIMALS. 2023 Jan;13(15):2544Read more>>
  • Zheng Ming-Hui. et al. Vascular wall microenvironment: exosomes secreted by adventitial fibroblasts induced vascular calcification. J NANOBIOTECHNOL. 2023 Dec;21(1):1-21Read more>>
  • Lin Xu. et al. Local injection of abaloparatide promotes mandibular condyle lengthening in adolescent rats via enhancing chondrogenesis and ossification. J ORAL REHABIL. 2023 SepRead more>>
VALIDATION IMAGES