bs-11445R [Primary Antibody]
NCF1/p47 phox(Ser359) Antibody
www.biossusa.com
[email protected]
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+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NCF1/p47 phox Ser359

Modification Site: Ser359

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 653361

Source: KLH conjugated synthetic phosphopeptide derived from human NCF1 around the phosphorylation site of Ser359

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 45


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Gangling, et al. "Limb Remote Ischemic Postconditioning Reduces Ischemia-Reperfusion Injury by Inhibiting NADPH Oxidase Activation and MyD88-TRAF6-P38MAP-Kinase Pathway of Neutrophils." International Journal of Molecular Sciences 17.12 (2016): 1971.Read more>>
  • Arifen, Nahida. et al. Sirtuin1 inhibitor attenuates hypertension in spontaneously hypertensive rats: role of Giα proteins and nitroxidative stress. J HYPERTENS. 2022 Jul;40(7):1314-1326Read more>>
  • Shen, Liang. et al. Angiotensin Type 2 Receptor Pharmacological Agonist Relieves Neurocognitive Deficits via Reducing Neuroinflammation and Microglial Engulfment of Dendritic Spines. J NEUROIMMUNE PHARM. 2022 Dec;:1-17Read more>>
  • Xin Guo. et al. Aspirin protects human trophoblast HTR-8/SVneo cells from H2O2-Induced oxidative stress via NADPH/ROS pathway. PLACENTA. 2023 Dec;144:55Read more>>
VALIDATION IMAGES

Lane 1: Rat Lymph node tissue lysates; Lane 2: Human Raji cell lysates; Lane 3: Human THP-1 cell lysate probed with phospho-NCF1 (Ser359) Polyclonal Antibody, Unconjugated (bs-11445R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.