bs-11492R [Primary Antibody]
UNC5B/UNC5H2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: UNC5B/UNC5H2

Immunogen Range: 188-260/945


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human UNC5B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The UNC5H family of proteins act as transmembrane receptors for netrin-1 and play a crucial role in axon guidance and migration of neural cells. In fact, UNC5H receptors express widely in cells that migrate, where they bind the G protein G Alpha 1-2 to inhibit G protein signaling. Additionally, UNC5H receptors induce apoptosis when cleaved by a caspase, producing an intracellular fragment containing a death domain, but this activity is blocked by the binding of netrin-1. The expression of UNC5H receptors is down-regulated in multiple cancers, including colorectal, breast, ovary, uterus, stomach, lung, and kidney cancers. Hence, in the absence of netrin-1, UNC5H receptors act as tumor suppressors by inhibiting anchorage-independent growth and invasion, but mutation of these receptors provides a potential mechanism for tumorigenicity. UNC5H2, also designated unc-5 homolog B or p53-regulated receptor for death and life protein 1 (p53RDL1) is highly expressed in brain with lower levels of expression observed in developing lung, cartilage, kidney and hematopoietic and immune tissues.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 101


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ko CL et al. Netrin-1 Dampens Hypobaric Hypoxia-Induced Lung Injury in Mice. High Alt Med Biol. 2019 Jul 22. Read more>>
  • Ding S et al. Macrophage-derived Netrin-1 participates in endometriosis-associated pain. Neurobiology of Disease.2019.Read more>>
  • Shaojie Ding. et al. Macrophage-derived netrin-1 contributes to endometriosis-associated pain. Ann Transl Med. 2021 Jan; 9(1): 29Read more>>
  • Shijie Wu. et al. High expression of UNC5B enhances tumor proliferation, increases metastasis, and worsens prognosis in breast cancer. Aging-Us. 2020 Sep 15; 12(17): 17079C17098Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UNC5B/UNC5H2 Polyclonal Antibody, Unconjugated (bs-11492R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nuclei.


Lane 1: U87MG cell lysates;Lane 2: HT1080 cell lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat cerebrum lysates probed with UNC5B Polyclonal Antibody, Unconjugated (bs-7537R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.