bs-11501R [Primary Antibody]
GFRA3/GDNF Receptor alpha 3 Antibody
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Host: Rabbit

Target Protein: GFRA3/GDNF Receptor alpha 3

Immunogen Range: 101-185/400


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human GFR alpha 3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Glial cell line-derived neurotrophic factor (GDNF) and the related neurotrophic factor neurturin (NTN) are potent survival factors for central and peripheral neurons. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGF Beta superfamily of growth factors. Three receptors for these factors, GFR Alpha-1 (also designated GDNFR-Alpha, RETL1 or TrnR-1), GFR Alpha-2 (also designated GDNFR-Beta, RETL2, NTNR-Alpha or TrnR-2) and GFR Alpha-3 have been identified. The receptors do not contain transmembrane domains and are attached to the cell membrane by glycosyl-phosphoinositol linkage. Both GFR Alpha-1 and GFR Alpha-2 have been shown to mediate the GDNF-dependent and NTN-dependent phosphorylation and activation of the tyrosine kinase Ret. GFR Alpha-3 is expressed only during development.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 38


Predicted Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Rat Spleen tissue lysates; Lane 4: Rat Stomach tissue lysates probed with GFR alpha 3 Polyclonal Antibody, Unconjugated (bs-11501R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.