bs-1152R [Primary Antibody]
ATP1b2/Na+K+ATPase Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ATP1b2/Na+K+ATPase

Immunogen Range: 201-290/290

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 482

Swiss Prot: P14415

Source: KLH conjugated synthetic peptide derived from human ATP1b2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Mediates cell adhesion of neurons and astrocytes, and promotes neurite outgrowth.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 33

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang HY et al. Occludin Endocytosis is Involved in Disruption of the Intestinal Epithelial Barrier in a Mouse Model of Alcoholic Steatohepatitis. J Dig Dis. 2019 Jul 12. Read more>>
  • Shiman Zuo. et al. Establishment of a novel mesenchymal stem cell-based regimen for chronic myeloid leukemia differentiation therapy. Cell Death Dis. 2021 Feb;12(2):1-15Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse colon labeled with Anti- ATP1b2/Na+K+ATPase Polyclonal Antibody, Unconjugated (bs-1152R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 rat kidney lysates L2 rat colon lysates probed with Anti ATP1b2/Na+K+ATPase Polyclonal Antibody, Unconjugated (bs-1152R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 33kD. Observed band size: 33kD.