bs-1153R [Primary Antibody]
Bid Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Bid

Immunogen Range: 102-195/195


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12122

Swiss Prot: P70444

Source: KLH conjugated synthetic peptide derived from mouse Bid

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 21


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Fan et al. Resveratrol induces autophagy-dependent apoptosis in HL-60 cells. (2018) BMC.Cancer. 18:581Read more>>
  • Guang\zhao Huang. et al. Screening and identification of autophagy\related biomarkers for oral squamous cell carcinoma (OSCC) via integrated bioinformatics analysis. 2021 Apr 09Read more>>
  • Tiantian Qin. et al. Luteolin combined with low-dose paclitaxel synergistically inhibits epithelialCmesenchymal transition and induces cell apoptosis on esophageal carcinoma in vitro and in vivo. 2021 Sep 07Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-Bid Polyclonal Antibody, Unconjugated (bs-1153R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bid) polyclonal Antibody, Unconjugated (bs-1153R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.