bs-11546R [Primary Antibody]
DUSP6 Polyclonal Antibody
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Host: Rabbit

Target Protein: DUSP6

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8642

Source: KLH conjugated synthetic peptide derived from human DUSP6

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Mitogen-activated protein (MAP) kinases are a large class of proteins involved in signal transduction pathways that are activated by a range of stimuli and mediate a number of physiological and pathological changes in the cell. Dual specificity phosphatases (DSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases. DSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK and p38. The members of the dual-specificity phosphatase protein family include MKP-1/CL100 (3CH134), VHR, PAC1, MKP-2, hVH-3 (B23), hVH-5, MKP-3, MKP-X, and MKP-4. Human MKP-3 maps to chromosome 12q22-q23 and encodes a 381 amino acid protein that specifically inactivates members of the ERK family and is expressed in a variety of tissues with the highest levels in heart and pancreas.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Guang Y., et al. "HCV induced reduction in miR‐181a impairs CD4+ T cell responses via over‐expression of DUSP6." Hepatology (2014).Read more>>
  • Rodrigues et al. Obesity Increases Mitogen-Activated Protein Kinase Phosphatase-3 Levels in the Hypothalamus of Mice. (2017) Front.Cell.Neurosci. 11:313Read more>>
  • Li et al. Hepatitis C virus-induced reduction in miR-181a impairs CD4(+) T-cell responses through overexpression of DUSP6. (2015) Hepatology. 61:1163-73Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti DUSP6/MKP-3 Polyclonal Antibody, Unconjugated (bs-11546R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DUSP6) Polyclonal Antibody, Unconjugated (bs-11546R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.