Host: Rabbit
Target Protein: PDE7B
Specificity: Due to the sequence similarity, there is a chance that this antibody will recognize PDE7A, but the sequence is not continuous.
Immunogen Range: 281-360/450
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 27115
Swiss Prot: Q9NP56
Source: KLH conjugated synthetic peptide derived from human PDE7B
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 52
Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Mouse cerebrum lysates probed with PDE7B Polyclonal Antibody, unconjugated (bs-11576R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
RSC96 cells probed with PDE7B Polyclonal Antibody, Unconjugated (bs-11576R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PDE7B Polyclonal Antibody, Unconjugated (bs-11576R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PDE7B Polyclonal Antibody, Unconjugated (bs-11576R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with PDE7B Polyclonal Antibody, Unconjugated (bs-11576R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.