bs-1164R [Primary Antibody]
ADFP Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ADFP

Immunogen Range: 31-130/422


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 298199

Source: KLH conjugated synthetic peptide derived from rat ADFP

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP) associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, was a major constituent of the globule surface and was present in a detergent-insoluble complex that contained stoichiometric amounts of butyrophilin and xanthine oxidase. ADFP (Adipophilin) occurs in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to certain cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Amrutkar, Manoj, et al. "Genetic Disruption of Protein Kinase STK25 Ameliorates Metabolic Defects in a Diet-Induced Type 2 Diabetes Model."Diabetes (2015).Read more>>
  • Zhao et al. Adipose differentiation‑related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation. (2017) Mol.Med.Re. 16:3079-3086Read more>>
  • Liu et al. Oxidized low-density lipoprotein activates adipophilin through ERK1/2 signal pathway in RAW264.7 cells. (2010) Acta.Biochim.Biophys.Sin.(Shanghai. 42:635-45Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human placenta labeled with Anti-ADFP Polyclonal Antibody, Unconjugated (bs-1164R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Mouse liver lysates probed with ADFP Polyclonal Antibody, Unconjugated (bs-1164R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.