bs-11655R [Primary Antibody]
ACAN Polyclonal Antibody
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Host: Rabbit

Target Protein: ACAN

Immunogen Range: 601-700/2415


Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P16112

Source: KLH conjugated synthetic peptide derived from Human Aggrecan

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The large chondroitin sulfate proteoglycan, aggrecan, is the predominant proteoglycan present in cartilage. Aggrecan is a member of the chondroitin sulphate proteoglycan family, which also includes versican/PG-M, neurocan and brevican. Aggrecan is a complex multidomain macromolecule that undergoes extensive processing and post-translational modification. Aggrecan in cartilage forms aggregates with hyaluronan and link protein, embedded in a collagen network. Aggrecan accounts for the compressive stiffness and resilience of the hyaline cartilage. Many forms of inflammatory arthritis are shown to be accompanied with aggrecan degradation and loss from the cartilage. Brevican is a brain proteoglycan of the aggrecan/versican/neurocan family. In the adult brain, the brevican core protein undergoes proteolytic cleavage and exists as a full-length form a carboxy-terminal fragment and an amino-terminal fragment.

Size: 100µl

Concentration: Lot Dependent

Applications: ELISA
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
IHC-P

Predicted Molecular Weight: 99


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tian, Fa‑Ming, et al. "Orally administered simvastatin partially preserves lumbar vertebral bone mass but not integrity of intervertebral discs in ovariectomized rats." Experimental and Therapeutic Medicine.Read more>>
  • Fang DP et al. Platelet‐rich plasma promotes the regeneration of cartilage engineered by mesenchymal stem cells and collagen hydrogel via the TGF‐β/SMAD signaling pathway. J Cell Physiol. 2019;1–11.Read more>>
  • Sinkemani A et al. Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions. Stem Cells Int.2020 Dec 23;2020:8882549.Read more>>
  • Carolina C. Zuliani. et al. Chondrogenesis of human amniotic fluid stem cells in Chitosan-Xanthan scaffold for cartilage tissue engineering. Sci Rep-Uk. 2021 Feb;11(1):1-9Read more>>
  • Mara Herrero-Herrero. et al. Influence of chemistry and fiber diameter of electrospun PLA, PCL and their blend membranes, intended as cell supports, on their biological behavior. Polym Test. 2021 Sep;:107364Read more>>
  • ?brahim Halilullah Erbay. et al. Bioengineering bone-on-a-chip model harnessing osteoblastic and osteoclastic resolution. ADV ENG MATER. 2022 DecRead more>>
  • Pinger Wang. et al. Chondroprotective Mechanism of <em>Eucommia ulmoides</em> Oliv.-<em>Glycyrrhiza uralensis</em> Fisch. Couplet Medicines in Knee Osteoarthritis via Experimental Study and Network Pharmacology Analysis. DRUG DES DEV THER. 2023 Feb;17:633-646Read more>>
  • Haiming Wang. et al. Low-frequency whole-body vibration can enhance cartilage degradation with slight changes in subchondral bone in mice with knee osteoarthritis and does not have any morphologic effect on normal joints. PLOS ONE. 2023 Aug;18(8):e0270074Read more>>
  • Ching-Yu Lee. et al. Development and functional evaluation of a hyaluronic acid coated nano-formulation with kaempferol as a novel intra-articular agent for Knee Osteoarthritis treatment. BIOMED PHARMACOTHER. 2024 Jun;175:116717Read more>>
  • Jianbo Xu. et al. Exploring the pharmacological mechanism of Glycyrrhiza uralensis against KOA through integrating network pharmacology and experimental assessment. J CELL MOL MED. 2024 May;28(9):e18319Read more>>
  • Han Yin. et al. Chondrocyte-derived apoptotic vesicles enhance stem cell biological function for the treatment of cartilage injury. CHEM ENG J. 2024 Aug;:154501Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded bone of mouse embryo labeled with Anti-CATSPER Polyclonal Antibody, Unconjugated (bs-2986R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Aggrecan Polyclonal Antibody, Unconjugated (bs-11655R)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Rat embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACAN) Polyclonal Antibody, Unconjugated (bs-11655R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACAN) Polyclonal Antibody, Unconjugated (bs-11655R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.