bs-1165R [Primary Antibody]
Beta catenin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Beta catenin

Immunogen Range: 661-781/781


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1499

Swiss Prot: P35222

Source: KLH conjugated synthetic peptide derived from human Beta catenin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 9.5/86


Cross Reactive Species: Human
Mouse
Rat
Pig
Zebrafish

Predicted Cross Reactive Species: Cow
Sheep
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xie M et al. Porcine milk exosome miRNAs protect intestinal epithelial cells against deoxynivalenol-induced damage. Biochem Pharmacol. 2020 May;175:113898.Read more>>
  • Lu, Juan, et al. "Expression of Wnt3a, Wnt10b, β-catenin and DKK1 in periodontium during orthodontic tooth movement in rats." Acta Odontologica Scandinavica (2015): 1-7.Read more>>
  • Yang, Ruicheng, et al. "Induction of VEGFA and Snail-1 by meningitic Escherichia coli mediates disruption of the blood-brain barrier." Oncotarget (2016).Read more>>
  • Meng, Ying, et al. "Silencing MARCH1 suppresses proliferation, migration and invasion of ovarian cancer SKOV3 cells via downregulation of NF-κB and Wnt/β-catenin pathways." Oncology Reports (2016).Read more>>
  • Hu, Jianguo, et al. "Ubiquitin E3 ligase MARCH7 promotes ovarian tumor growth." Oncotarget 6.14 (2015): 12174.Read more>>
  • Zheng, Jian, et al. "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." Journal of Neurosurgery (2016): 1-9.Read more>>
  • Zhu, Afang, et al. "Suppression of Wnt5a, but not Wnts, relieves chronic post-thoracotomy pain via anti-inflammatory modulation in rats." Biochemical and Biophysical Research Communications (2017).Read more>>
  • Qi et al. Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss. (2016) Peer. 4:e2624Read more>>
  • Zou et al. Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway. (2018) EBioMedicine. 31:287-298Read more>>
  • Ma W et al. A vanillin derivative suppresses the growth of HT29 cells through the Wnt/β-catenin signaling pathway. Eur J Pharmacol. 2019 Apr 15;849:43-49. Read more>>
  • Xia W et al. Inhibition of MRP4 alleviates sepsis-induced acute lung injury in rats. Int Immunopharmacol. 2019 Apr 14;72:211-217.Read more>>
  • Li M et al. Plant MIR156 Regulates Intestinal Growth in Mammals by Targeting the Wnt/β-catenin Pathway. Am J Physiol Cell Physiol. 2019 Jun 5. Read more>>
  • Pan J et al. lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/β-catenin signaling. Mol Cancer. 2020 Jan 15;19(1):9. Read more>>
  • Yang J et al. MicroRNA-708 represses hepatic stellate cells activation and proliferation by targeting ZEB1 through Wnt/β-catenin pathway. Eur J Pharmacol. 2020 Mar 15;871:172927. Read more>>
  • Xie M et al. Porcine milk exosome miRNAs protect intestinal epithelial cells against deoxynivalenol-induced damage. Biochem Pharmacol. 2020 May;175:113898.Read more>>
  • Chen Y et al. PLK1 regulates hepatic stellate cell activation and liver fibrosis through Wnt/\catenin signalling pathway. J Cell Mol Med. 2020 Jul;24(13):7405-7416.Read more>>
  • Chunyu Zhang et al. WISP1 promotes bovine MDSC differentiation via recruitment of ANXA1 for the regulation of the TGF- signalling pathway. Mol Cell Biochem. 2020 Jul;470(1-2):215-227.Read more>>
  • Hua Zhang #et al. The Expression of Tripartite Motif Protein 36 and -Catenin Correlates with the Prognosis of Esophageal Cancer. Gastroenterol Res Pract. 2020 Jul 31;2020:7641761.Read more>>
  • Shi M et al. MicroRNA-27a targets Sfrp1 to induce renal fibrosis in diabetic nephropathy by activating Wnt/-Catenin signalling. Biosci Rep. 2020 Jun 26;40(6):BSR20192794.Read more>>
  • Luo Liwen. et al. Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes. Front Cell Dev Biol. 2021 Mar;9:482Read more>>
  • Qingxin Fan. et al. Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/-Catenin Signaling in 3T3-L1 Adipocytes. Med Sci Monitor. 2021; 27: e928619-1Ce928619-1Read more>>
  • Hai Zhao. et al. Oxidative stress caused by a dysregulated Wnt/-catenin signalling pathway is involved in abnormal placenta formation in pregnant mice with chronic fatigue syndrome. Zygote. 2020 Oct;:1-8Read more>>
  • Kuangen Zhang. et al. Phosphorylated LASS2 inhibits prostate carcinogenesis via negative regulation of Wnt/-catenin signaling. 2021 Apr 14Read more>>
  • Xiaoyue Guan. et al. Crosstalk between Wnt/-catenin signaling and NF-B signaling contributes to apical periodontitis. Int Immunopharmacol. 2021 Sep;98:107843Read more>>
  • Lihong Zhang. et al. Disruption of anchoring junctions in the?testes of experimental varicocele rats. Exp Ther Med. 2021 Aug;22(2):1-11Read more>>
  • Zhang, Zilong. et al. Circ_FBLN1 promotes the proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by regulating let-7i-5p/FZD4 axis and Wnt/-catenin pathway. 2021 Aug 23Read more>>
  • Ling Xie. et al. Suppression of GOLM1 by EGCG through HGF/HGFR/AKT/GSK-3/-catenin/c-Myc signaling pathway inhibits cell migration of MDA-MB-231. Food Chem Toxicol. 2021 Nov;157:112574Read more>>
  • Zheng Wu. et al. FOXD3 suppresses epithelialCmesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22Read more>>
  • Xinkun Shen. et al. Improvement of aqueous stability and anti-osteoporosis properties of Zn-MOF coatings on titanium implants by hydrophobic raloxifene. Chem Eng J. 2021 Oct;:133094Read more>>
  • Liuqi Shi. et al. A dual-functional chitosan derivative platform for fungal keratitis. Carbohyd Polym. 2022 Jan;275:118762Read more>>
  • Jin Q. et al. Integrated Transcriptome and Multiple Activated Pathways in Endometrial Cancer.. Front Genet. 2021 Dec;12:680331-680331Read more>>
  • Zhao, Gang. et al. LINC02381, a sponge of miR-21, weakens osteogenic differentiation of hUC-MSCs through KLF12-mediated Wnt4 transcriptional repression. 2021 Nov 15Read more>>
VALIDATION IMAGES

Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Beta catenin Polyclonal Antibody (bs-1165R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Beta catenin Polyclonal Antibody (bs-1165R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Formalin-fixed and paraffin embedded human esophageal cancer labeled with Anti-β-catenin/Catenin β Polyclonal Antibody, Unconjugated (bs-1165R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Beta catenin Polyclonal Antibody, Unconjugated(bs-1165R) at 1:200 followed by conjugation to the secondary antibody,


Formalin-fixed and paraffin embedded rat skin labeled with Anti-β-catenin/Catenin β Polyclonal Antibody, Unconjugated (bs-1165R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin-embedded rat brain labeled with Anti-β-catenin/Catenin β Polyclonal Antibody, Unconjugated(bs-1165R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.