bs-11667R [Primary Antibody]
PET112L Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PET112L

Immunogen Range: 101-200/557


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human PET112L

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

PET112L is the human homolog of the S. cerevisiae COX assembly protein pet112, a protein that is believed to play an important role in the translation of mitochondrial genes. PET112L, also known as HSPC199 or Glu-ADT subunit B (glutamyl-tRNA(Gln) amidotransferase subunit B), is a 557 amino acid protein belonging to the gatB/gatE family of proteins (GatB subfamily) and is believed to play a role in energy metabolism. Localizing to mitochondria, PET112L is expressed in tissues such as heart and muscle, which exhibit high rates of oxidative phosphorylation. The gene encoding PET112L is overexpressed in recurrent ependymoma.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 58


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Cow

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse kidney lysates probed with PET112L Polyclonal Antibody, unconjugated (bs-11667R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Mouse heart lysates probed with PET112L Polyclonal Antibody, unconjugated (bs-11667R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.