bs-11731R [Primary Antibody]
LC3A/B Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LC3A/B

Immunogen Range: 31-121/121

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84557

Swiss Prot: Q9H492

Source: KLH conjugated synthetic peptide derived from human LC3A/B

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months..

Background: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Cow
Sheep
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cui, Xiaodong, et al. "Changes of intracellular Ca2+ in quercetin-induced autophagy progression." Acta Biochimica et Biophysica Sinica (2015): gmv096.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-LC3A/B Polyclonal Antibody, Unconjugated (bs-11731R) at 1:200 followed by conjugation to the secondary antibody and DAB staining