bs-1175R [Primary Antibody]
SHBG Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SHBG

Specificity: This antibody will not recognize Isoform 2 or 4 of SHBG.

Immunogen Range: 301-402/402


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6462

Swiss Prot: P04278

Source: KLH conjugated synthetic peptide derived from human SHBG

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 44


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Longfei Xiao. et al. Dihydrotestosterone through blockade of TGF-/Smad signaling mediates the anti-fibrosis effect under hypoxia in canine Sertoli cells. J Steroid Biochem. 2022 Feb;216:106041Read more>>
  • Longfei Xiao. et al. Curcumin Ameliorates Age-Induced Tight Junction Impaired in Porcine Sertoli Cells by Inactivating the NLRP3 Inflammasome through the AMPK/SIRT3/SOD2/mtROS Signaling Pathway. OXID MED CELL LONGEV. 2023;2023:1708251Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat uterus tissue labeled with Anti-SHBG Polyclonal Antibody, Unconjugated(bs-1175R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 mouse testis lysates, L2 mouse liver lysates probed with Anti-SHBG Polyclonal Antibody, Unconjugated (bs-1175R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band and observed band size: 44kD