bs-1179R [Primary Antibody]
CTLA-4/CD152 Polyclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: CTLA4

Immunogen Range: 183-223/223


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1493

Source: KLH conjugated synthetic peptide derived from human CTLA-4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

Size: 100uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yu et al. Cytotoxic T lymphocyte antigen 4 expression in human breast cancer: implications for prognosis. (2015) Cancer.Immunol.Immunothe. 64:853-60Read more>>
  • Lan et al. Cytotoxic T lymphocyte associated antigen 4 expression predicts poor prognosis in luminal B HER2-negative breast cancer. (2018) Oncol.Lett. 15:5093-5097Read more>>
  • Zengwei Chen. et al. Quantitative analysis of multiple breast cancer biomarkers using DNA-PAINT. ANAL METHODS-UK. 2022 AugRead more>>
  • Meiying Song. et al. Pathogenic Th17 cell-mediated liver fibrosis contributes to resistance to PD-L1 antibody immunotherapy in hepatocellular carcinoma. INT IMMUNOPHARMACOL. 2024 Mar;129:111601Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse colon labeled with Rabbit Anti CTLA-4 Polyclonal Antibody, Unconjugated (bs-1179R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded mouse tumor labeled with Anti-CTLA-4 Polyclonal Antibody, Unconjugated(bs-1179R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.DAPI was used to stain the cell nucleus.


Formalin-fixed and paraffin-embedded human liver cancer labeled with Anti-CTLA-4 Polyclonal Antibody, Unconjugated(bs-1179R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.DAPI was used to stain the cell nucleus.


Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Spleen tissue lysates; Lane 3: Rat Lung tissue lysates probed with CTLA4 Polyclonal Antibody, Unconjugated (bs-1179R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Thymus tissue lysates; Lane 3: Mouse Lymph node tissue lysates; Lane 4: Rat Spleen tissue lysates; Lane 5: Rat Thymus tissue lysates; Lane 6: Rat Lymph node tissue lysates; Lane 7: Human Raji cell lysates; Lane 8: Human K562 cell lysates probed with CTLA4 Polyclonal Antibody, Unconjugated (bs-1179R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.