bs-11842R [Primary Antibody]
Frizzled 9/CD349 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Frizzled 9/CD349

Specificity: This antibody may have slight cross reactivity with Frizzled 10.

Immunogen Range: 185-270/591


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8326

Swiss Prot: O00144

Source: KLH conjugated synthetic peptide derived from human Frizzled 9/CD349

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 62


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Vasyliev, R. G., et al. "Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications." Experimental Oncology 39.3 (2017): 171-180.Read more>>
VALIDATION IMAGES

Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human MCF-7 cell lysates probed with Frizzled 9 Polyclonal Antibody, Unconjugated (bs-11842R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.