bs-1189R [Primary Antibody]
Gastrin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Gastrin

Immunogen Range: 77-92/101


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2520

Swiss Prot: P01350

Source: KLH conjugated synthetic peptide derived from human Gastrin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 2.1/3.9/6/8


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kenta Kawasaki. et al. An Organoid Biobank of Neuroendocrine Neoplasms Enables Genotype-Phenotype Mapping. Cell. 2020 Nov;183:142Read more>>
  • Yildiz, M.. et al. The Effect of Chronic Intermittent Cold Exposure on Gastrin-, Somatostatin-, Secretin-, and Serotonin-Containing Cells in the Small Intestine of Rats. Biol Bull+. 2021 Dec;48(3):S95-S106Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human gastric parietal cells labeled with Anti-Gastrin Polyclonal Antibody, Unconjugated (bs-1189R) followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded mouse stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Gastrin Polyclonal Antibody, Unconjugated (bs-1189R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining


Paraformaldehyde-fixed, paraffin embedded (rat stomach ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gastrin) Polyclonal Antibody, Unconjugated (bs-1189R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.