bs-11994R [Primary Antibody]
Phosphoserine/threonine Antibody
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Host: Rabbit

Target Protein: Phosphoserine/threonine

Modification Site: serine + threonine

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic phosphopeptide containing a Phosphoserine and a Phosphothreonine

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity. The (Ser/Thr) Phe Antibody is a powerful tool for discovery of new proteins containing this important regulatory motif.

Size: 100uL

Concentration: 1ug/ul

Applications: IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Cross Reactive Species: Human
Others

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li et al. MAD2L2 inhibits colorectal cancer growth by promoting NCOA3 ubiquitination and degradation. (2018) Mol.Oncol. 12:391-405Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Serine/Threonine (p-Ser/p-Thr) Polyclonal Antibody, Unconjugated (bs-11994R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Serine/Threonine (p-Ser/p-Thr) Polyclonal Antibody, Unconjugated (bs-11994R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Serine/Threonine (p-Ser/p-Thr) Polyclonal Antibody, Unconjugated (bs-11994R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Serine/Threonine (p-Ser/p-Thr) Polyclonal Antibody, Unconjugated (bs-11994R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Serine/Threonine (p-Ser/p-Thr) Polyclonal Antibody, Unconjugated (bs-11994R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.