bs-12061R [Primary Antibody]
GABRA2/GABA A Receptor alpha 2 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GABRA2/GABA A Receptor alpha 2

Immunogen Range: 185-280/451


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2555

Swiss Prot: P47869

Source: KLH conjugated synthetic peptide derived from human GABRA2/GABA A Receptor alpha 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 48


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sancho-Alonso, Mara. et al. Hyperammonemia Enhances GABAergic Neurotransmission in Hippocampus: Underlying Mechanisms and Modulation by Extracellular cGMP. Mol Neurobiol. 2022 Mar;:1-18Read more>>
  • Yaiza M. Arenas. et al. Enhanced BDNF and TrkB Activation Enhance GABA Neurotransmission in Cerebellum in Hyperammonemia. INT J MOL SCI. 2022 Jan;23(19):1177Read more>>
VALIDATION IMAGES

Mouse brain lysates probed with Rabbit Anti-GABRA2/GABA A Receptor alpha 2 Polyclonal Antibody, Unconjugated (bs-12061R ) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.


Formalin-fixed and paraffin embedded rat brain labeled with Anti GABRA2/GABA A Receptor alpha 2 Polyclonal Antibody, Unconjugated (bs-12061R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


RSC96 cells probed with GABRA2/GABA A Receptor alpha 2 Antibody, Unconjugated (bs-12061R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).