bs-1206R [Primary Antibody]
GLI1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GLI1

Immunogen Range: 601-700/1106

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2735

Swiss Prot: P08151

Source: KLH conjugated synthetic peptide derived from human GLI1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months..

Background: Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sato Y et al. Blockade of Hedgehog Signaling Attenuates Biliary Cystogenesis in the Polycystic Kidney (PCK) Rat.Am J Pathol. 2018 Oct;188(10):2251-2263. Read more>>
  • Zhang, Qiang, et al. "Serotonin Receptor 2C and Insulin Secretion." PloS one 8.1 (2013): e54250.Read more>>
  • Yue, Yongjian, et al. "Aberrant activation of Hedgehog pathway in Nasopharyngeal carcinoma." Clinical and Experimental Medicine (2012): 1-8.Read more>>
  • Fan, Hai-Xia, et al. "Sonic hedgehog signaling may promote invasion and metastasis of oral squamous cell carcinoma by activating MMP-9 and E-cadherin expression." Medical Oncology 31.7 (2014): 1-8.Read more>>
  • Ma, Zhenkun, et al. "Silibinin induces apoptosis through inhibition of the mTOR-GLI1-BCL2 pathway in renal cell carcinoma." Oncology Reports.Read more>>
  • Long, B., et al. "Targeting GLI1 Suppresses Cell Growth and Enhances Chemosensitivity in CD34+ Enriched Acute Myeloid Leukemia Progenitor Cells." Cellular Physiology and Biochemistry 38.4 (2016): 1288-1302.Read more>>
  • Yang, Huan, et al. "Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats." Stem Cell Research & Therapy 8.1 (2017): 267.Read more>>
  • Fan et al. Doxorubicin combined with low intensity ultrasound suppresses the growth of oral squamous cell carcinoma in culture and in xenografts. (2017) J.Exp.Clin.Cancer.Re. 36:163Read more>>
  • Du et al. Targeting the SMO oncogene by miR-326 inhibits glioma biological behaviors and stemness. (2015) Neuro.Oncol. 17:243-53Read more>>
  • Liu et al. Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis. (2014) Oncotarget. 5:11681-94Read more>>
  • Men et al. LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration. (2015) Sci.Rep. 5:16232Read more>>
  • Chen X et al. Hesperetin derivative attenuates CCl4-induced hepatic fibrosis and inflammation by Gli-1-dependent mechanisms. Int Immunopharmacol. 2019 Aug 29;76:105838. Read more>>
  • Guo Q et al. Baicalin improves the in-vitro developmental capacity of pig embryos by inhibiting apoptosis, regulating mitochondrial activity and activating sonic hedgehog signaling. Mol Hum Reprod. 2019 Sep 1;25(9):538-549.Read more>>
  • Sato Y et al. Blockade of Hedgehog Signaling Attenuates Biliary Cystogenesis in the Polycystic Kidney (PCK) Rat.Am J Pathol. 2018 Oct;188(10):2251-2263. Read more>>
  • Xiaolan You. et al. Galectin-1 promotes vasculogenic mimicry in gastric adenocarcinoma via the Hedgehog/GLI signaling pathway.. Aging-Us. 2020 Nov 15; 12(21): 21837C21853Read more>>
  • Mi Zhang. et al. 2\adrenergic receptor signaling drives prostate cancer progression by targeting the Sonic hedgehog\Gli1 signaling activation. Prostate. 2020 Nov;80(15):1328-134Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human glioma labeled with Anti-GLI1/ZFP5 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, DAB staining.


HepG2 lysates probed with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


HeLa cells were stained with GLI1 Polyclonal Antibody, Unconjugated(bs-1206R) at 1:50 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.


HepG2 cells were stained with GLI1 Polyclonal Antibody, Unconjugated(bs-1206R) at 1:50 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.