VALIDATION IMAGES
Human k562 lysates probed with Rabbit Anti-P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat ovary tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat ovary tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Human HL60 cell lysates probed with Rabbit Anti-P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded mouse embryo tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with
P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Ovary lysates probed with P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HL60 cell lysates; Lane 2: K562 cell lysates probed with P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (bs-12074R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (bs-12074R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Ovary tissue lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human U87MG cell lysates probed with P2Y9 Polyclonal Antibody, Unconjugated (bs-12074R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.