bs-1208R [Primary Antibody]
ADAMTS1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ADAMTS1

Specificity: Contains 3 TSP type-1 domains

Immunogen Range: 851-967/967


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11504

Swiss Prot: P97857

Source: KLH conjugated synthetic peptide derived from mouse ADAMTS1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. Has angiogenic inhibitor activity (By similarity). Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 106


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Cow
Sheep
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Anti-ADAMTS1 Polyclonal Antibody, Unconjugated(bs-1208R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-ADAMTS1 Polyclonal Antibody, Unconjugated (bs-1208R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin-embedded rat tongue labeled with Anti-ADAMTS1 Polyclonal Antibody, Unconjugated(bs-1208R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


Lane 1: Human Hela cell Lysates; Lane 2: Human 293T cell Lysates; Lane 3: Human U87-MG cell Lysates; Lane 4: Human U251 cell Lysates. Probed with ADAMTS1 polyclonal Antibody, unconjugated (bs-1208R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.