bs-12095R [Primary Antibody]
GRID1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Rabbit

Target Protein: GRID1

Immunogen Range: 501-600/1009


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2894

Swiss Prot: Q9ULK0

Source: KLH conjugated synthetic peptide derived from human GRID1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Glutamate receptors mediate most excitatory neurotransmissions in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are divided into two categories, namely NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors consist of seven structurally related subunits, designated GluR-1 to -7, and are primarily responsible for fast excitatory neurotransmissions carried out by glutamate. GluR-delta 1 (Glutamate receptor delta-1 subunit), also known as GRID1, is a multi-pass membrane protein that belongs to the kainate/AMPA receptor family and is expressed primarily in the brain. Localized to the cell junction and the postsynaptic cell membrane, GluR-delta 1 functions as a glutamate receptor that regulates synaptic transmissions in the central nervous system (CNS) and is thought to play an important role in synaptic plasticity. Defects in the gene encoding GluR-delta 1 are associated with schizophrenia, a chronic and severe brain disorder.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 110


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Sheep
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lloyd Brian A. et al. Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus. NAT COMMUN. 2023 Aug;14(1):1-22Read more>>
VALIDATION IMAGES

Hela cells blocked with 1 X PBS containing 5% BSA. Cells were incubated with GRID1 Polyclonal Antibody (bs-12095R) at 1:50 dilution (2ug/1x10^6 Cells) (green) compared with an unlabeled cells (black), secondary only (bs-0295G-APC; blue) and isotype control (bs-0295P; orange).


K562 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GRID1 Polyclonal Antibody(bs-12095R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).