bs-1210R [Primary Antibody]
BRCA2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: BRCA2

Immunogen Range: 21-130/3418

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 675

Swiss Prot: P51587

Source: KLH conjugated synthetic peptide derived from human BRCA2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and DSS1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180).

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 384

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Juliet Goldsmithet al. Ribosome profiling reveals a functional role for autophagy in mRNA translational control. Commun Biol. 2020 Jul 17;3(1):388.Read more>>
  • Elkafas H et al. Vitamin D3 Ameliorates DNA Damage Caused by Developmental Exposure to Endocrine Disruptors in the Uterine Myometrial Stem Cells of Eker Rats. Cells. 2020 Jun 12;9(6):1459.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human breast carcinoma labeled Anti-BRCA2 Polyclonal Antibody, Unconjugated (bs-1210R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Image provided by the Independent Validation Program badge 29807.\n\nLane 1: MCF7 cell lysate Lane 2:CAPAN1 cell lysates probed with Rabbit Anti-BRCA2 Polyclonal Antibody, Unconjugated (bs-1210R) at 1:200 for 10 hours at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 37˚C.