bs-12147R [Primary Antibody]
ZIC5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ZIC5

Immunogen Range: 501-600/663


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human ZIC5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Zic5 (zinc finger protein of the cerebellum 5) is a C2H2 zinc finger transcription factor that influences development of the neural crest. Zic family members are abundant in developing and adult cerebellum. Zic family members are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. Zic5 is closely linked to Zic2, a related family member on chromosome 13.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 68


Predicted Cross Reactive Species: Human
Mouse
Rat
Dog
Cow
Sheep
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tan, Yi-Fan. et al. AR-regulated ZIC5 contributes to the aggressiveness of prostate cancer. CELL DEATH DISCOV. 2022 Sep;8(1):1-15Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ZIC5) Polyclonal Antibody, Unconjugated (bs-12147R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.