bs-12153R [Primary Antibody]
TXLNB Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TXLNB

Specificity: This may have some cross-reactivity with TXLNA and TXLNG.

Immunogen Range: 331-430/684


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 167838

Swiss Prot: Q8N3L3

Source: KLH conjugated synthetic peptide derived from human TXLNB

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Beta-taxilin promotes nerve regeneration and may be involved in intracellular vesicle transport. Expressed predominantly in cardiac and skeletal muscle, beta-taxilin binds to the coiled coil region of the syntaxin family members STX1A, STX3A, and STX4A. Beta- and gamma-taxilins, bind to the alpha subunit of the nascent polypeptide-associated complex (NAC) and affect its nuclear distribution, suggesting that the taxilin family is involved not only in the translational process through its interaction with NAC but also in the transcriptional process through its interaction with alphaNAC alone.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 76


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded human muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with TXLNB Polyclonal Antibody, Unconjugated (bs-12153R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Mouse skeletal muscle lysate probed with Anti TXLNB Polyclonal Antibody, Unconjugated (bs-12153R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 76kD. Observed band size: 76kD