Host: Rabbit
Target Protein: ST18/Znf387
Immunogen Range: 451-550/1047
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9705
Swiss Prot: O60284
Source: KLH conjugated synthetic peptide derived from human ST18/Znf387
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 115
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Dog
Sheep
Horse
For research use only. Not intended for diagnostic or therapeutic use.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-ST18/Znf387 Polyclonal Antibody, Unconjugated (bs-12188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse kidney were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ST18/Znf387 Polyclonal Antibody(bs-12188R)at 1:50dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
U87mg cell lysates probed with ST18/Znf387Polyclonal Antibody, Unconjugated (bs-12188R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed withST18/Znf387 Polyclonal Antibody, Unconjugated (bs-12188R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.