bs-1219R [Primary Antibody]
GRP78 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GRP78

Immunogen Range: 251-360/654


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3309

Swiss Prot: P11021

Source: KLH conjugated synthetic peptide derived from human GRP78

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC1, probably to facilitate the release of DNAJC1 from its substrate.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 78


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang P et al. Loss of CD36 impairs hepatic insulin signaling by enhancing the interaction of PTP1B with IR. FASEB J. 2020 Apr;34(4):5658-5672.Read more>>
  • Wei, W, et al. "Excessive fluoride induces endoplasmic reticulum stress and interferes enamel proteinases secretion." Environmental Toxicology 28 (2013):Read more>>
  • Du, Weiyan, et al. "The Expression and Significance of Angiopoietin-2 and Glucose Regulated Protein78 in Colorectal Cancer." Cancer Cell Research (2016).Read more>>
  • Chen, Li, et al. "Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid." Endocrine (2016): 1-13.Read more>>
  • Tian, Jing, Rong Liu, and Quanxin Qu. "Role of endoplasmic reticulum stress on cisplatin resistance in ovarian carcinoma." Oncology Letters.Read more>>
  • Xia, Dan, et al. "Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress." Cell Death and Disease 8.7 (2017).Read more>>
  • Tian et al. Role of endoplasmic reticulum stress on cisplatin resistance in ovarian carcinoma. (2017) Oncol.Let. 13:1437-1443Read more>>
  • Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.Read more>>
  • Zhao,et al.Osteopontin contributes to effective neutrophil recruitment, IL-1β production and apoptosis in Aspergillus fumigatus keratitis.(2018) Immunology and Cell Biology. 96:401-412.Read more>>
  • Wang,et al.Chaihulonggumulitang Shows Psycho-cardiology Therapeutic Effects on Acute Myocardial Infarction by Enhancing Bone Marrow Mesenchymal Stem Cells Mobilization.(2018) Scientific Reports. 8:3724.Read more>>
  • Jiang H et al.Strontium Alleviates Endoplasmic Reticulum Stress in a Nonalcoholic Fatty Liver Disease Model.(2018)J Med Food. Nov 17. Read more>>
  • Zuo L et al. PI3-kinase/Akt pathway-regulated membrane transportation of acid-sensing ion channel1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF-induced HSC Activation. J Cell Mol Med. 2019 Apr 2. Read more>>
  • Zuo Y et al. Preoperative vitamin-rich carbohydrate loading alleviates postoperative cognitive dysfunction in aged rats. Behav Brain Res. 2019 Nov 5;373:112107.Read more>>
  • Cui J et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress-and Ca2. Oxid Med Cell Longev. 2019 Jan 9;2019:2593742. Read more>>
  • Lan J et al. Endoplasmic reticulum stress induces liver cells apoptosis after brain death by suppressing the phosphorylation of protein phosphatase 2A. Molecular Medicine Reports 21.2 (2020): 567-574.Read more>>
  • Zhu YP et al. Unification of Opposites between Two Antioxidant Transcription Factors Nrf1 and Nrf2 in Mediating Distinct Cellular Responses to the Endoplasmic Reticulum Stressor Tunicamycin. Antioxidants (Basel). 2019 Dec 19;9(1). pii: E4. Read more>>
  • Zhou L et al. KLF15-activating Twist2 ameliorated hepatic steatosis by inhibiting inflammation and improving mitochondrial dysfunction via NF-κB-FGF21 or SREBP1c-FGF21 pathway. FASEB J. 2019 Oct 24:fj201901347RR.Read more>>
  • Yang P et al. Loss of CD36 impairs hepatic insulin signaling by enhancing the interaction of PTP1B with IR. FASEB J. 2020 Apr;34(4):5658-5672.Read more>>
  • Ling Li. et al. Avian leukosis virus subgroup J and reticuloendotheliosis virus coinfection induced TRIM62 regulation of the actin cytoskeleton. J Vet Sci. 2020 May; 21(3): e49Read more>>
  • Xuejiao Zhou. et al. The novel ALK inhibitor ZX\29 induces apoptosis through inhibiting ALK and inducing ROS\mediated endoplasmic reticulum stress in Karpas299 cells. 2020 Nov 02Read more>>
  • Derya Karabulut. et al. Thymoquinone attenuates doxorubicin\cardiotoxicity in rats. J Biochem Mol Toxic. 2021 Jan;35(1):e22618Read more>>
  • Yong-yi Dai. et al. Effects of exogenous vitamin D on endoplasmic reticulum stress-related factors in the vitamin D deficiency rats with cerebral ischemia. Neurosci Lett. 2021 Jul;:136115Read more>>
  • Emin Kaymak. et al. Thymoquinone has a neuroprotective effect against inflammation, oxidative stress, and endoplasmic reticulum stress in the brain cortex, medulla, and hippocampus due to doxorubicin. 2021 Aug 15Read more>>
  • Ozturk, E.. et al. Evaluation by different mechanisms of the protective effects of vitamin B12 on methotrexate nephrotoxicity. 2021 Oct 16Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:400 overnight at 4°C, DAB staining.


Mouse placenta probed with Rabbit Anti-GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.


Image provided by One World Lab validation program. SMMC7721 cells probed with Rabbit Anti-GRP78 Polyclonal Antibody (bs-1219R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Image provided by One World Lab validation program. PC3 cells probed with Rabbit Anti-GRP78 Polyclonal Antibody (bs-1219R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti-GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


U87MG cell lysates probed with GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Rat Liver lysates probed with GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Rat Spleen lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Human U-87MG cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human MDA-MB-231 cell lysates; Lane 7: Human A431 cell lysates probed with GRP78 Polyclonal Antibody, Unconjugated (bs-1219R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.