Host: Rabbit
Target Protein: ZNF740
Immunogen Range: 101-193/193
Clonality: Polyclonal
Isotype: IgG
Source: KLH conjugated synthetic peptide derived from Human ZNF740
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 22
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Cow
Sheep
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded human cervical carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-ZNF740 Polyclonal Antibody, Unconjugated (bs-12227R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Mouse kidney lysates probed with ZNF740 Polyclonal Antibody, Unconjugated (bs-12227R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.