bs-12258R [Primary Antibody]
FUT2 Polyclonal Antibody
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Host: Rabbit

Target Protein: FUT2

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human FUT2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values. FUT2 is expressed on the surface of several human tumor cell lines such as BEL-7404, SPC-A-1, and SGC-7901.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhengchang Wu. et al. Insight into mechanisms of pig lncRNA FUT3-AS1 regulating E. coli F18-bacterial diarrhea. PLOS PATHOG. 2022 Jun;18(6):e1010584Read more>>
  • Zhang Dan-Dan. et al. Butyrate protects the intestinal barrier by upregulating Fut2 expression via MEK4-JNK signaling pathway activation. PEDIATR RES. 2024 Aug;:1-1Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human stomach labeled with Anti-FUT2 Polyclonal Antibody, Unconjugated (bs-12258R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (FUT2) Polyclonal Antibody, Unconjugated (bs-12258R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.