bs-12280R [Primary Antibody]
Stella/DPPA3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Stella/DPPA3

Immunogen Range: 101-159/159


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 359787

Swiss Prot: Q6W0C5

Source: KLH conjugated synthetic peptide derived from human Stella/DPPA3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 18


Cross Reactive Species: Mouse
Pig

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wasielak, M., et al. "Zygote arrest 1 (ZAR-1), nucleoplasmin 2 (NPM2), and developmentally associated protein 3 (DPPA3) mRNA profiles throughout porcine embryo development in vitro." Theriogenology (2016).Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-Stella/DPPA3 Polyclonal Antibody, Unconjugated (bs-12280R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse testis lysates probed with Stella Polyclonal Antibody, Unconjugated (bs-12280R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.