bs-12334R [Primary Antibody]
GCNT1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GCNT1

Immunogen Range: 201-260/428


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2650

Source: KLH conjugated synthetic peptide derived from human GCNT1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

GCNT1 is expressed in a variety of cell types, including lymphocytes and mucin-producing cells. Specifically, GCNT1 expression in leukocytes regulates the synthesis of core 2 O-glycans that carry sialyl-Lewis x (sLex) oligosaccharides, which confer high affinity binding to Selectins. Downregulation of Selectin ligand expression levels has been shown to inhibit tissue infiltration. Therefore, GCNT1 represents a potential drug target for the treatment of inflammatory disorders and other pathologies involving Selectins.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 50


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Cerebrum lysates probed with GCNT1 Polyclonal Antibody, Unconjugated (bs-12334R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: U87MG cell lysates probed with GCNT1 Polyclonal Antibody, Unconjugated (bs-12334R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Rat Lymph node tissue lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human TT cell lysates; Lane 4: Human U87MG cell lysates; Lane 5: Human MCF-7 cell lysates probed with GCNT1 Polyclonal Antibody, Unconjugated (bs-12334R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.