Host: Rabbit
Target Protein: EDA/Ectodermal dysplasia protein
Immunogen Range: 101-200/391
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1896
Swiss Prot: Q92838
Source: KLH conjugated synthetic peptide derived from human Ectodysplasin-A, membrane form/secreted form
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 41
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Cow
For research use only. Not intended for diagnostic or therapeutic use.
- Sisto, Margherita, et al. "Downstream activation of NF‐κB in the EDA‐A1/EDAR signalling in Sjögrens syndrome and its regulation by the ubiquitin‐editing enzyme A20." Clinical & Experimental Immunology (2016).Read more>>
- Sisto et al. Downstream activation of NF-κB in the EDA-A1/EDAR signalling in Sjögren's syndrome and its regulation by the ubiquitin-editing enzyme A20. (2016) Clin.Exp.Immunol. Read more>>
Lane 1: mouse heart lysates probed with EDA Polyclonal Antibody (bs-12347R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDA) Polyclonal Antibody, Unconjugated (bs-12347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Skin tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Rat Embryo tissue lysates; Lane 5: Rat Skin tissue lysates; Lane 6: Rat Stomach tissue lysates; Lane 7: Rat Kidney tissue lysates probed with EDA Polyclonal Antibody, Unconjugated (bs-12347R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.