bs-1234R [Primary Antibody]
RASSF1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RASSF1

Immunogen Range: 145-260/340


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11186

Swiss Prot: Q9NS23

Source: KLH conjugated synthetic peptide derived from human RASSF1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF1 stimulation. Isoform A interacts with CDC2, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 39


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Wen-bin, et al. "CpG island hypermethylation of multiple tumor suppressor genes associated with loss of their protein expression during rat lung carcinogenesis induced by 3-methylcholanthrene and diethylnitrosamine." Biochemical and biophysical research communications 402.3 (2010): 507.Read more>>
  • Klacz, Jakub, et al. "Decreased expression of RASSF1A tumor suppressor gene is associated with worse prognosis in clear cell renal cell carcinoma."International Journal of Oncology.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-RASSF1A Polyclonal Antibody, Unconjugated (bs-1234R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-RASSF1A Polyclonal Antibody, Unconjugated (bs-1234R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin-embedded human oral squamous cell labeled with Anti-RASSF1A Polyclonal Antibody, Unconjugated(bs-1234R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.


Formalin-fixed and paraffin-embedded human oral squamous cell labeled with Anti-RASSF1A Polyclonal Antibody, Unconjugated(bs-1234R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FTIC)used at 1:200 dilution for 40 minutes at 37°C.