bs-12352R [Primary Antibody]
GSC Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GSC

Immunogen Range: 151-257/257


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 145258

Source: KLH conjugated synthetic peptide derived from human GSC

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Goosecoid is a homeodomain transcription factor with DNA binding specificity identical to that of the anterior morphogen 'bicoid' in Drosophila.During mouse embryogenesis, GSC influences development of the lower mandible and its associated musculature, including the tongue, the nasal cavity, and the nasal pits, as well as components of the inner ear and the external auditory meatus. The GSC gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 28


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Cow
Sheep
Pig
Chicken
Rabbit
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-GSC Polyclonal Antibody, Unconjugated (bs-12352R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-GSC Polyclonal Antibody, Unconjugated (bs-12352R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.