bs-12367R [Primary Antibody]
TAZ Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TAZ

Immunogen Range: 1-100/400


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25937

Swiss Prot: Q9GZV5

Source: KLH conjugated synthetic peptide derived from human TAZ

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun, Lidan, et al. "Prognostic impact of TAZ and beta-catenin expression in adenocarcinoma of the esophagogastric junction." Diagnostic Pathology 9.1 (2014): 125.Read more>>
  • Ukita, Mayumi, et al. "Sclerostin Enhances Adipocyte Differentiation in 3T3‐L1 Cells." Journal of Cellular Biochemistry (2015).Read more>>
  • Yang Xi. et al. circ0005027 Acting as a ceRNA Affects the Malignant Biological Behavior of Hypopharyngeal Squamous Cell Carcinoma by Modulating miR-548c-3p/CDH1 Axis. BIOCHEM GENET. 2023 Nov;:1-16Read more>>
  • Yiwei Wang. et al. M2 macrophages promote subconjunctival fibrosis through YAP/TAZ signalling. ANN MED. 2024 Feb 09Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TAZ Polyclonal Antibody, Unconjugated (bs-12367R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Lane 1: mouse lung lysates probed with \tTAZ Polyclonal Antibody, Unconjugated (bs-12367R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TAZ Polyclonal Antibody, Unconjugated (bs-12367R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TAZ Polyclonal Antibody(bs-12367R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Lane 1: Mouse Kidney lysates; Lane 2: Rat Kidney lysates probed with TAZ Polyclonal Antibody, Unconjugated (bs-12367R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.