bs-12386R [Primary Antibody]
LMX1A Polyclonal Antibody
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Host: Rabbit

Target Protein: LMX1A

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4009

Source: KLH conjugated synthetic peptide derived from human LMX1A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

LMX1A belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1A functions in the nucleus as a transcriptional activator to the insulin gene promoter. In the developing embryo, LMX1A is expressed along the neuraxis and leads to the development of the roof plate of the vertebrae. Two isoforms of LMX1A exist due to alternative splicing. Isoform 1 represents the full length protein and is expressed in many tissues including fetal brain, but is absent in heart, liver, spleen and testis. The second isoform, designated LMX1A-4AB, lacks amino acids 1-249 and is expressed in testis.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sato et al. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells. (2016) Sci.Rep. 6:31063Read more>>
VALIDATION IMAGES

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates; Lane 5: Human HeLa cell lysates; Lane 6: Human MCF-7 cell lysates probed with LMX1A Polyclonal Antibody, Unconjugated (bs-12386R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.