DATASHEET
Host:
Rabbit
Target Protein:
KAT3A / CBP
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
1387
Source:
KLH conjugated synthetic peptide derived from human KAT3A/CBP
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression, even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.
Conjugation:
AbBy Fluor-647
Excitation/ Emission:
650nm/665nm
Size:
100µL
Concentration:
1ug/ul
Applications:
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)
Flow-Cyt(1ug/Test)
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.